Rotavirus strain surveillance for three years following the introduction of rotavirus vaccine into Belém, Brazil
Guerra, Sylvia de Fátima dos Santos
Linhares, Alexandre da Costa
Mascarenhas, Joana D'Arc Pereira
Justino, Maria Cleonice Aguiar
Soares, Luana da Silva
Müller, Elza Caroline
The monovalent human rotavirus (RV) vaccine, RIX4414 (Rotarix , GlaxoSmithKline Biologicals) was introduced into Brazil's Expanded Program on Immunization in March 2006. One year after vaccine introduction, the G2P strain was found to be predominant, with an apparent extinction of many non-G2 strains. This study investigated the diversity of circulating strains in the three years following RIX4414 introduction. Between May 2008 and May 2011, stool samples were collected from children aged =12 weeks who were hospitalized for severe lab confirmed RV-gastroenteritis (=3 liquid or semi-liquid motions over a 24-h period for >14 days, requiring =1 overnight hospital stay and intravenous rehydration therapy) in Belém, Brazil. RV-gastroenteritis was detected by ELISA and the G- and P-types were determined by RT-PCR assays. During the first year of surveillance nucleotide sequencing was used for typing those samples not previously typed by RT-PCR. A total of 1,726 of 10,030 severe gastroentertis hospitalizations (17.2 per cent) were due to severe RVGE. G2P was detected in 57.2 per cent of circulating strains over the whole study period, however it predominated during the first 20 months from May 2008 to January 2009. G1P increased in the last part of the study period from May 2010 to May 2011 and represented 36.6 per cent (112/306) of the circulating strains. G2P was the predominant RV strain circulating during the first 20 months of the study, followed by G1P. These findings probably reflect a natural fluctuation in RV strains over time, rather than a vaccine-induced selective pressure.
xmlui.dri2xhtml.METS-1.0.item-citationGUERRA, Sylvia de Fátima dos Santos et al. Rotavirus strain surveillance for three years following the introduction of rotavirus vaccine into Belém, Brazil. Journal of Medical Virology, v. 87, n. 8, p. 1303-1310, Apr. 2015.
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